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gelred nucleic acid gel stain  (Biotium)


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    Structured Review

    Biotium gelred nucleic acid gel stain
    Gelred Nucleic Acid Gel Stain, supplied by Biotium, used in various techniques. Bioz Stars score: 97/100, based on 3998 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gelred+nucleic+acid+gel+stain/GelRed+Nucleic+Acid+Gel+Stain/pmc13690978-275-1-0
    Average 97 stars, based on 3998 article reviews
    gelred nucleic acid gel stain - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Staining:

    Article Title: Establishment of Reverse Transcription Polymerase Chain Reaction Method for Quantification of Residual Host Cell RNA in Recombinant Plasmid Drugs
    Article Snippet: Reagents: Total RNA extraction kit (Shanghai Promega Biological Products Co., Ltd. (Shanghai, China), catalog number: LS1040), LB Broth (without sugar) (Guangdong Huankai Microbial Sci.&Tech. .. Co., Ltd., (Guangzhou, China), catalog number: 028324), Agarose (Biowest (Nuaillé, France), distributed by Shanghai Baygene Biotechnology Co., Ltd. (Shanghai, https://doi.org/10.3390/molecules31203670 China), catalog number: BY-R0100), 50 × TAE Buffer (Jiangsu Cowin Biotech Co., Ltd. (Taizhou, China), catalog number: CW0663S), GelRed Nucleic Acid Gel Stain (BIOTIUM (Fremont, CA, USA), catalog number: 41003), 6 × Loading Buffer (Takara Bio Inc. (Kusatsu, Shiga, Japan), catalog number: 9156), BS15000 DNA Marker (Bosun Life (Beijing, China), catalog number: BS1103), DNase I, RNase-free (Nanjing Vazyme Biotechnology Co., Ltd. (Nanjing, China), catalog number: EN401-01), AccurSTART II U+ One Step RT-qPCR Probe Kit (containing 5 × One Step U+ Mix, One Step U+ Enzyme Mix, and 50 × ROX Reference Dye) (Nanjing Vazyme Biotechnology Co., Ltd., catalog number: Q233-02), DNase/RNasefree deionized water (Tiangen Biochemical Technology Co., Ltd. (Beijing, China), catalog number: 1109285). .. Primers and probe were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).

    Article Title: Influence of Hepatocellular Senescence on Serum Amyloid A Expression and Response to Proinflammatory Cytokines under Steatotic Conditions.
    Article Snippet: .. The amplified PCR products were separated by 2% agarose gel electrophoresis and visualized with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, U.S.A.). .. Band intensities were quantified using ImageJ software.

    Article Title: Gene Expression of Neuropeptides in the Blood Cells of the Common Carp, Cyprinus carpio
    Article Snippet: .. PCR products were analyzed by 1.8% agarose gel electrophoresis, and the amplicons were stained with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, USA). ..

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Primer as designed in Basic Protocol (e.g., ‐21M13 F TGTAAAACGACGGCCAGT and M13 R CAGGAAACAGCTATGAC; https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/304/660/2grhsrmkb.pdf )— KAPA2G Robust HotStart ReadyMix (Roche, cat. no. KK5702) UltraPure DNase/RNase‐Free Distilled Water (Invitrogen, cat. no. 10977015) Agarose (Low‐EEO/Multi‐Purpose/Molecular Biology Grade) (Fisher BioReagents, cat. no. BP160‐100 Ampure XP beads (Beckman Coulter, cat. no. A63881) 80% ethanol ONT Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies, cat. no. SQK‐NBD114.24) UltraPure 10X TBE BUFFER (Invitrogen, cat. no. 15581‐028) GelRed Nucleic Acid Gel Stain (Biotium, cat. no. 41003) 1kb Plus DNA Ladder (Invitrogen, cat. no. 10787018) Mini Gel Caster (Bio‐Rad, cat. no. 1704422) Wide Mini‐Sub Cell GT Gel Apparatus (Bio‐Rad, cat. no. 1704468) Sterile tubes, 1.5 mL (SSI Bio, cat. no. 1210‐S0) Invitrogen Qubit 1X dsDNA Broad Range (BR) Assay Flongle Adapter (Oxford Nanopore Technologies, cat. no. FLGIntSP) Dorado [Oxford Nanopore Technologies ( GitHub ‐ Nanoporetech/Dorado: Oxford Nanopore's Basecaller · GitHub , June 8, 2026)] PowerPac Basic Power Supply (Bio‐Rad, cat. no. 1645050) Gel Doc XR+ Molecular Imager (Bio‐Rad) 8‐Strip PCR Tubes & Clear, Dome Caps, 0.2 mL (SSI Bio, cat. no. 3132‐00) MinION sequencer (Oxford Nanopore Technologies, cat. no. MinION Mk1D) Samtools (H. Li et al., ) Integrative Genomics Viewer (IGV) [Broad Institute (Thorvaldsdóttir et al., )] FLAIR (Full‐Length Alternative Isoform analysis of RNA; Tang et al., ) Intron‐prospector ( GitHub ‐ Diekhans/Intron‐Prospector: Identify Putative Introns in RNA‐Seq Alignments · GitHub , August 18, 2026) .. Amplify cDNA amplicon using the following thermocycler conditions: Note: We use Roche Kappa Robust 2G ReadyMix with the ‐21M13F and M13R primers, but primers will be specific to the sequences designed in Step 1, and many other DNA polymerase systems are available.

    Article Title: Host-Specific Circulation and Molecular Diversity of Babesia , Hepatozoon and Bartonella Species Among Wild Carnivores in Northwestern Italy.
    Article Snippet: Amplifications for TBPs were performed using Wonder Taq DNA Polymerase (Euroclone, Milan, Italy) and a T100TM Thermal Cycler (Bio-Rad, Hercules, CA, USA). .. PCR products were visualized using a UV transilluminator after electrophoresis migration on 2% agarose gel stained with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, USA) in 1× standard tris-acetate-EDTA (TAE) buffer. .. SharpMass 100 Plus Ladder (Euroclone, Milan, Italy) was used as a molecular weight marker.

    Article Title: Reciprocal coupling between EMT and BMP morphogen signalling drives collective cell invasion.
    Article Snippet: .. Approximately 300–500 ng of each sample was mixed with loading dye and resolved on a 1% agarose gel in 1× TAE containing GelRed Nucleic Acid Gel Stain (Biotium). ..

    Article Title: Molecular and morphological characterization of hard ticks (Acari: Ixodidae) infesting rodents in Southern Iran: evidence of a cryptic Haemaphysalis species.
    Article Snippet: .. PCR products were separated on 2% agarose gel by electrophoresis and stained with GelRed nucleic acid gel stain (Biotium, USA) for visualization under a BioDoc gel documentation System. ..

    Article Title: Probe‐Free, Primer‐Programmed Amplicon Encoding Enables Nanopore‐Based Multiplex Detection With Deep Learning Decoding
    Article Snippet: Additional reagents, such as TaqMan Fast Virus 1‐Step Multiplex Master Mix (#5555532) and Tris‐EDTA buffer, were acquired from Thermo Fisher and Sigma‐Aldrich. .. Biotium GelRed Nucleic Acid Gel Stain, 10 000× (#NC9594719), was purchased from Fisher Scientific. .. RPA reaction kits and enzymes (#TALQBAS01) were provided by TwistDx Ltd (Cambridge, UK).

    Marker:

    Article Title: Establishment of Reverse Transcription Polymerase Chain Reaction Method for Quantification of Residual Host Cell RNA in Recombinant Plasmid Drugs
    Article Snippet: Reagents: Total RNA extraction kit (Shanghai Promega Biological Products Co., Ltd. (Shanghai, China), catalog number: LS1040), LB Broth (without sugar) (Guangdong Huankai Microbial Sci.&Tech. .. Co., Ltd., (Guangzhou, China), catalog number: 028324), Agarose (Biowest (Nuaillé, France), distributed by Shanghai Baygene Biotechnology Co., Ltd. (Shanghai, https://doi.org/10.3390/molecules31203670 China), catalog number: BY-R0100), 50 × TAE Buffer (Jiangsu Cowin Biotech Co., Ltd. (Taizhou, China), catalog number: CW0663S), GelRed Nucleic Acid Gel Stain (BIOTIUM (Fremont, CA, USA), catalog number: 41003), 6 × Loading Buffer (Takara Bio Inc. (Kusatsu, Shiga, Japan), catalog number: 9156), BS15000 DNA Marker (Bosun Life (Beijing, China), catalog number: BS1103), DNase I, RNase-free (Nanjing Vazyme Biotechnology Co., Ltd. (Nanjing, China), catalog number: EN401-01), AccurSTART II U+ One Step RT-qPCR Probe Kit (containing 5 × One Step U+ Mix, One Step U+ Enzyme Mix, and 50 × ROX Reference Dye) (Nanjing Vazyme Biotechnology Co., Ltd., catalog number: Q233-02), DNase/RNasefree deionized water (Tiangen Biochemical Technology Co., Ltd. (Beijing, China), catalog number: 1109285). .. Primers and probe were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).

    Quantitative RT-PCR:

    Article Title: Establishment of Reverse Transcription Polymerase Chain Reaction Method for Quantification of Residual Host Cell RNA in Recombinant Plasmid Drugs
    Article Snippet: Reagents: Total RNA extraction kit (Shanghai Promega Biological Products Co., Ltd. (Shanghai, China), catalog number: LS1040), LB Broth (without sugar) (Guangdong Huankai Microbial Sci.&Tech. .. Co., Ltd., (Guangzhou, China), catalog number: 028324), Agarose (Biowest (Nuaillé, France), distributed by Shanghai Baygene Biotechnology Co., Ltd. (Shanghai, https://doi.org/10.3390/molecules31203670 China), catalog number: BY-R0100), 50 × TAE Buffer (Jiangsu Cowin Biotech Co., Ltd. (Taizhou, China), catalog number: CW0663S), GelRed Nucleic Acid Gel Stain (BIOTIUM (Fremont, CA, USA), catalog number: 41003), 6 × Loading Buffer (Takara Bio Inc. (Kusatsu, Shiga, Japan), catalog number: 9156), BS15000 DNA Marker (Bosun Life (Beijing, China), catalog number: BS1103), DNase I, RNase-free (Nanjing Vazyme Biotechnology Co., Ltd. (Nanjing, China), catalog number: EN401-01), AccurSTART II U+ One Step RT-qPCR Probe Kit (containing 5 × One Step U+ Mix, One Step U+ Enzyme Mix, and 50 × ROX Reference Dye) (Nanjing Vazyme Biotechnology Co., Ltd., catalog number: Q233-02), DNase/RNasefree deionized water (Tiangen Biochemical Technology Co., Ltd. (Beijing, China), catalog number: 1109285). .. Primers and probe were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).

    Amplification:

    Article Title: Influence of Hepatocellular Senescence on Serum Amyloid A Expression and Response to Proinflammatory Cytokines under Steatotic Conditions.
    Article Snippet: .. The amplified PCR products were separated by 2% agarose gel electrophoresis and visualized with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, U.S.A.). .. Band intensities were quantified using ImageJ software.

    Polymerase Chain Reaction:

    Article Title: Influence of Hepatocellular Senescence on Serum Amyloid A Expression and Response to Proinflammatory Cytokines under Steatotic Conditions.
    Article Snippet: .. The amplified PCR products were separated by 2% agarose gel electrophoresis and visualized with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, U.S.A.). .. Band intensities were quantified using ImageJ software.

    Article Title: Gene Expression of Neuropeptides in the Blood Cells of the Common Carp, Cyprinus carpio
    Article Snippet: .. PCR products were analyzed by 1.8% agarose gel electrophoresis, and the amplicons were stained with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, USA). ..

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Primer as designed in Basic Protocol (e.g., ‐21M13 F TGTAAAACGACGGCCAGT and M13 R CAGGAAACAGCTATGAC; https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/304/660/2grhsrmkb.pdf )— KAPA2G Robust HotStart ReadyMix (Roche, cat. no. KK5702) UltraPure DNase/RNase‐Free Distilled Water (Invitrogen, cat. no. 10977015) Agarose (Low‐EEO/Multi‐Purpose/Molecular Biology Grade) (Fisher BioReagents, cat. no. BP160‐100 Ampure XP beads (Beckman Coulter, cat. no. A63881) 80% ethanol ONT Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies, cat. no. SQK‐NBD114.24) UltraPure 10X TBE BUFFER (Invitrogen, cat. no. 15581‐028) GelRed Nucleic Acid Gel Stain (Biotium, cat. no. 41003) 1kb Plus DNA Ladder (Invitrogen, cat. no. 10787018) Mini Gel Caster (Bio‐Rad, cat. no. 1704422) Wide Mini‐Sub Cell GT Gel Apparatus (Bio‐Rad, cat. no. 1704468) Sterile tubes, 1.5 mL (SSI Bio, cat. no. 1210‐S0) Invitrogen Qubit 1X dsDNA Broad Range (BR) Assay Flongle Adapter (Oxford Nanopore Technologies, cat. no. FLGIntSP) Dorado [Oxford Nanopore Technologies ( GitHub ‐ Nanoporetech/Dorado: Oxford Nanopore's Basecaller · GitHub , June 8, 2026)] PowerPac Basic Power Supply (Bio‐Rad, cat. no. 1645050) Gel Doc XR+ Molecular Imager (Bio‐Rad) 8‐Strip PCR Tubes & Clear, Dome Caps, 0.2 mL (SSI Bio, cat. no. 3132‐00) MinION sequencer (Oxford Nanopore Technologies, cat. no. MinION Mk1D) Samtools (H. Li et al., ) Integrative Genomics Viewer (IGV) [Broad Institute (Thorvaldsdóttir et al., )] FLAIR (Full‐Length Alternative Isoform analysis of RNA; Tang et al., ) Intron‐prospector ( GitHub ‐ Diekhans/Intron‐Prospector: Identify Putative Introns in RNA‐Seq Alignments · GitHub , August 18, 2026) .. Amplify cDNA amplicon using the following thermocycler conditions: Note: We use Roche Kappa Robust 2G ReadyMix with the ‐21M13F and M13R primers, but primers will be specific to the sequences designed in Step 1, and many other DNA polymerase systems are available.

    Article Title: Host-Specific Circulation and Molecular Diversity of Babesia , Hepatozoon and Bartonella Species Among Wild Carnivores in Northwestern Italy.
    Article Snippet: Amplifications for TBPs were performed using Wonder Taq DNA Polymerase (Euroclone, Milan, Italy) and a T100TM Thermal Cycler (Bio-Rad, Hercules, CA, USA). .. PCR products were visualized using a UV transilluminator after electrophoresis migration on 2% agarose gel stained with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, USA) in 1× standard tris-acetate-EDTA (TAE) buffer. .. SharpMass 100 Plus Ladder (Euroclone, Milan, Italy) was used as a molecular weight marker.

    Article Title: Molecular and morphological characterization of hard ticks (Acari: Ixodidae) infesting rodents in Southern Iran: evidence of a cryptic Haemaphysalis species.
    Article Snippet: .. PCR products were separated on 2% agarose gel by electrophoresis and stained with GelRed nucleic acid gel stain (Biotium, USA) for visualization under a BioDoc gel documentation System. ..

    Agarose Gel Electrophoresis:

    Article Title: Influence of Hepatocellular Senescence on Serum Amyloid A Expression and Response to Proinflammatory Cytokines under Steatotic Conditions.
    Article Snippet: .. The amplified PCR products were separated by 2% agarose gel electrophoresis and visualized with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, U.S.A.). .. Band intensities were quantified using ImageJ software.

    Article Title: Gene Expression of Neuropeptides in the Blood Cells of the Common Carp, Cyprinus carpio
    Article Snippet: .. PCR products were analyzed by 1.8% agarose gel electrophoresis, and the amplicons were stained with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, USA). ..

    Article Title: Host-Specific Circulation and Molecular Diversity of Babesia , Hepatozoon and Bartonella Species Among Wild Carnivores in Northwestern Italy.
    Article Snippet: Amplifications for TBPs were performed using Wonder Taq DNA Polymerase (Euroclone, Milan, Italy) and a T100TM Thermal Cycler (Bio-Rad, Hercules, CA, USA). .. PCR products were visualized using a UV transilluminator after electrophoresis migration on 2% agarose gel stained with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, USA) in 1× standard tris-acetate-EDTA (TAE) buffer. .. SharpMass 100 Plus Ladder (Euroclone, Milan, Italy) was used as a molecular weight marker.

    Article Title: Reciprocal coupling between EMT and BMP morphogen signalling drives collective cell invasion.
    Article Snippet: .. Approximately 300–500 ng of each sample was mixed with loading dye and resolved on a 1% agarose gel in 1× TAE containing GelRed Nucleic Acid Gel Stain (Biotium). ..

    Article Title: Molecular and morphological characterization of hard ticks (Acari: Ixodidae) infesting rodents in Southern Iran: evidence of a cryptic Haemaphysalis species.
    Article Snippet: .. PCR products were separated on 2% agarose gel by electrophoresis and stained with GelRed nucleic acid gel stain (Biotium, USA) for visualization under a BioDoc gel documentation System. ..

    Sterility:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Primer as designed in Basic Protocol (e.g., ‐21M13 F TGTAAAACGACGGCCAGT and M13 R CAGGAAACAGCTATGAC; https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/304/660/2grhsrmkb.pdf )— KAPA2G Robust HotStart ReadyMix (Roche, cat. no. KK5702) UltraPure DNase/RNase‐Free Distilled Water (Invitrogen, cat. no. 10977015) Agarose (Low‐EEO/Multi‐Purpose/Molecular Biology Grade) (Fisher BioReagents, cat. no. BP160‐100 Ampure XP beads (Beckman Coulter, cat. no. A63881) 80% ethanol ONT Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies, cat. no. SQK‐NBD114.24) UltraPure 10X TBE BUFFER (Invitrogen, cat. no. 15581‐028) GelRed Nucleic Acid Gel Stain (Biotium, cat. no. 41003) 1kb Plus DNA Ladder (Invitrogen, cat. no. 10787018) Mini Gel Caster (Bio‐Rad, cat. no. 1704422) Wide Mini‐Sub Cell GT Gel Apparatus (Bio‐Rad, cat. no. 1704468) Sterile tubes, 1.5 mL (SSI Bio, cat. no. 1210‐S0) Invitrogen Qubit 1X dsDNA Broad Range (BR) Assay Flongle Adapter (Oxford Nanopore Technologies, cat. no. FLGIntSP) Dorado [Oxford Nanopore Technologies ( GitHub ‐ Nanoporetech/Dorado: Oxford Nanopore's Basecaller · GitHub , June 8, 2026)] PowerPac Basic Power Supply (Bio‐Rad, cat. no. 1645050) Gel Doc XR+ Molecular Imager (Bio‐Rad) 8‐Strip PCR Tubes & Clear, Dome Caps, 0.2 mL (SSI Bio, cat. no. 3132‐00) MinION sequencer (Oxford Nanopore Technologies, cat. no. MinION Mk1D) Samtools (H. Li et al., ) Integrative Genomics Viewer (IGV) [Broad Institute (Thorvaldsdóttir et al., )] FLAIR (Full‐Length Alternative Isoform analysis of RNA; Tang et al., ) Intron‐prospector ( GitHub ‐ Diekhans/Intron‐Prospector: Identify Putative Introns in RNA‐Seq Alignments · GitHub , August 18, 2026) .. Amplify cDNA amplicon using the following thermocycler conditions: Note: We use Roche Kappa Robust 2G ReadyMix with the ‐21M13F and M13R primers, but primers will be specific to the sequences designed in Step 1, and many other DNA polymerase systems are available.

    Qubit:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Primer as designed in Basic Protocol (e.g., ‐21M13 F TGTAAAACGACGGCCAGT and M13 R CAGGAAACAGCTATGAC; https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/304/660/2grhsrmkb.pdf )— KAPA2G Robust HotStart ReadyMix (Roche, cat. no. KK5702) UltraPure DNase/RNase‐Free Distilled Water (Invitrogen, cat. no. 10977015) Agarose (Low‐EEO/Multi‐Purpose/Molecular Biology Grade) (Fisher BioReagents, cat. no. BP160‐100 Ampure XP beads (Beckman Coulter, cat. no. A63881) 80% ethanol ONT Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies, cat. no. SQK‐NBD114.24) UltraPure 10X TBE BUFFER (Invitrogen, cat. no. 15581‐028) GelRed Nucleic Acid Gel Stain (Biotium, cat. no. 41003) 1kb Plus DNA Ladder (Invitrogen, cat. no. 10787018) Mini Gel Caster (Bio‐Rad, cat. no. 1704422) Wide Mini‐Sub Cell GT Gel Apparatus (Bio‐Rad, cat. no. 1704468) Sterile tubes, 1.5 mL (SSI Bio, cat. no. 1210‐S0) Invitrogen Qubit 1X dsDNA Broad Range (BR) Assay Flongle Adapter (Oxford Nanopore Technologies, cat. no. FLGIntSP) Dorado [Oxford Nanopore Technologies ( GitHub ‐ Nanoporetech/Dorado: Oxford Nanopore's Basecaller · GitHub , June 8, 2026)] PowerPac Basic Power Supply (Bio‐Rad, cat. no. 1645050) Gel Doc XR+ Molecular Imager (Bio‐Rad) 8‐Strip PCR Tubes & Clear, Dome Caps, 0.2 mL (SSI Bio, cat. no. 3132‐00) MinION sequencer (Oxford Nanopore Technologies, cat. no. MinION Mk1D) Samtools (H. Li et al., ) Integrative Genomics Viewer (IGV) [Broad Institute (Thorvaldsdóttir et al., )] FLAIR (Full‐Length Alternative Isoform analysis of RNA; Tang et al., ) Intron‐prospector ( GitHub ‐ Diekhans/Intron‐Prospector: Identify Putative Introns in RNA‐Seq Alignments · GitHub , August 18, 2026) .. Amplify cDNA amplicon using the following thermocycler conditions: Note: We use Roche Kappa Robust 2G ReadyMix with the ‐21M13F and M13R primers, but primers will be specific to the sequences designed in Step 1, and many other DNA polymerase systems are available.

    Analysis:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Primer as designed in Basic Protocol (e.g., ‐21M13 F TGTAAAACGACGGCCAGT and M13 R CAGGAAACAGCTATGAC; https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/304/660/2grhsrmkb.pdf )— KAPA2G Robust HotStart ReadyMix (Roche, cat. no. KK5702) UltraPure DNase/RNase‐Free Distilled Water (Invitrogen, cat. no. 10977015) Agarose (Low‐EEO/Multi‐Purpose/Molecular Biology Grade) (Fisher BioReagents, cat. no. BP160‐100 Ampure XP beads (Beckman Coulter, cat. no. A63881) 80% ethanol ONT Native Barcoding Kit 24 V14 (Oxford Nanopore Technologies, cat. no. SQK‐NBD114.24) UltraPure 10X TBE BUFFER (Invitrogen, cat. no. 15581‐028) GelRed Nucleic Acid Gel Stain (Biotium, cat. no. 41003) 1kb Plus DNA Ladder (Invitrogen, cat. no. 10787018) Mini Gel Caster (Bio‐Rad, cat. no. 1704422) Wide Mini‐Sub Cell GT Gel Apparatus (Bio‐Rad, cat. no. 1704468) Sterile tubes, 1.5 mL (SSI Bio, cat. no. 1210‐S0) Invitrogen Qubit 1X dsDNA Broad Range (BR) Assay Flongle Adapter (Oxford Nanopore Technologies, cat. no. FLGIntSP) Dorado [Oxford Nanopore Technologies ( GitHub ‐ Nanoporetech/Dorado: Oxford Nanopore's Basecaller · GitHub , June 8, 2026)] PowerPac Basic Power Supply (Bio‐Rad, cat. no. 1645050) Gel Doc XR+ Molecular Imager (Bio‐Rad) 8‐Strip PCR Tubes & Clear, Dome Caps, 0.2 mL (SSI Bio, cat. no. 3132‐00) MinION sequencer (Oxford Nanopore Technologies, cat. no. MinION Mk1D) Samtools (H. Li et al., ) Integrative Genomics Viewer (IGV) [Broad Institute (Thorvaldsdóttir et al., )] FLAIR (Full‐Length Alternative Isoform analysis of RNA; Tang et al., ) Intron‐prospector ( GitHub ‐ Diekhans/Intron‐Prospector: Identify Putative Introns in RNA‐Seq Alignments · GitHub , August 18, 2026) .. Amplify cDNA amplicon using the following thermocycler conditions: Note: We use Roche Kappa Robust 2G ReadyMix with the ‐21M13F and M13R primers, but primers will be specific to the sequences designed in Step 1, and many other DNA polymerase systems are available.

    Electrophoresis:

    Article Title: Host-Specific Circulation and Molecular Diversity of Babesia , Hepatozoon and Bartonella Species Among Wild Carnivores in Northwestern Italy.
    Article Snippet: Amplifications for TBPs were performed using Wonder Taq DNA Polymerase (Euroclone, Milan, Italy) and a T100TM Thermal Cycler (Bio-Rad, Hercules, CA, USA). .. PCR products were visualized using a UV transilluminator after electrophoresis migration on 2% agarose gel stained with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, USA) in 1× standard tris-acetate-EDTA (TAE) buffer. .. SharpMass 100 Plus Ladder (Euroclone, Milan, Italy) was used as a molecular weight marker.

    Article Title: Molecular and morphological characterization of hard ticks (Acari: Ixodidae) infesting rodents in Southern Iran: evidence of a cryptic Haemaphysalis species.
    Article Snippet: .. PCR products were separated on 2% agarose gel by electrophoresis and stained with GelRed nucleic acid gel stain (Biotium, USA) for visualization under a BioDoc gel documentation System. ..

    Migration:

    Article Title: Host-Specific Circulation and Molecular Diversity of Babesia , Hepatozoon and Bartonella Species Among Wild Carnivores in Northwestern Italy.
    Article Snippet: Amplifications for TBPs were performed using Wonder Taq DNA Polymerase (Euroclone, Milan, Italy) and a T100TM Thermal Cycler (Bio-Rad, Hercules, CA, USA). .. PCR products were visualized using a UV transilluminator after electrophoresis migration on 2% agarose gel stained with GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA, USA) in 1× standard tris-acetate-EDTA (TAE) buffer. .. SharpMass 100 Plus Ladder (Euroclone, Milan, Italy) was used as a molecular weight marker.



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